- Clb5 Clb6 Mutants
- clb5∆ clb6∆
- TAB6-1 clb5∆ clb6∆
- cdc20∆ clb5∆
- cdc20∆ pds1∆ clb5∆
- CLB2-db∆ clb5∆
- CLB2-db∆ clb5∆ in galactose
- cln1∆ cln2∆ clb5∆ clb6∆
- CLB5-db∆
- CLB5-db∆ sic1∆
- CLB5-db∆ pds1∆
- CLB5-db∆ pds1∆ cdc20∆
- GAL-CLB5
- GAL-CLB5 cdh1∆
- GAL-CLB5 sic1∆
- GAL-CLB5-db∆
- cln1∆ cln2∆ cln3∆ multi-copy CLB5
- cln1∆ cln2∆ cln3∆ GAL-CLB5
CLB5-db∆ sic1∆
debug: ,
test user =
test db =
Simulation:
Change of parameters: kdb5"=0, ksc1'=ksc1"=0.
Arrest: It dies after the first cycle, Ori not relicensed.
Experiments:
Jacobson, M.D., Gray, S., Yuste-Rojas, M. and Cross, F.R. (2000). Testing cyclin specificity in the exit from mitosis. Mol. Cell. Biol. 20:4483-4493.
[Abstract] [Article]
[Abstract] [Article]
Wasch, R. and Cross, F. (2002). APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for mitotic exit. Nature 418:556-562.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Wasch, Fig. 2. It has similar phenotype as sic1∆ cdh1∆ being semi-lethal on glucose medium. Mutant cell arrests with replicated but un-separated DNA without long mitotic spindle. It showa a high rate of loss for plasmids containing single replication origin, but the rate of loss is significantly decreased for multi-origin plasmids. This indicates that the mutant is not blocked from mitotic exit, but it has problems with origin usage.
Comments: In simulation, the mutant is able to exit the first mitosis, but dies because of DNA replication problem. [ORI] (grey line) is not re-licensed after the first mitosis.